Device

Part:BBa_K142074:Design

Designed by: Julius Rabl   Group: iGEM08_ETH_Zurich   (2008-10-29)


lac-controlled GFP-LVA generator with constitutive lacI expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1137
    Illegal NheI site found at 1160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

We put the LacI generator behind the lac-controlled expression cassette to minimze readthrough.



Source

Subcloned from BioBricks using standard molecular biology techniques.


References